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Bovine Pituitary Extract (BPE) Non-Sterile

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Bovine Pituitary Extract (BPE)

Non-Sterile Crude Preparation in Normal Saline
0.45 um Filter Clarified
US or NZ Origin

Bovine Pituitary Extract (BPE)
50008-25 25ml
50008-50 50ml
50008-100 100ml

BPE continues to be the most successful bovine derived extract to date and has allowed cell culture scientists to investigate and study normal primary cell types, including stem cells, non-fibroblast cells, and epithelial cells in proliferating cultures. BPE is a highly concentrated source of putative mitogens and growth factors beneficial for serum- free or low- serum containing media. J R Scientific’s (JRS) method of preparation is based on the original formulation determined in Dr. Richard Ham’s laboratory. Our product is prepared in normal saline and formulated to contain up to 20 mg/mL active protein. Growth tests performed on human mammary epithelial cells, epidermal keratinocytes, prostatic and bronchial epithelial cells indicate an optimum concentration range of 25-50 ug/ml depending on objectives. JRS recommends utilization of a growth assay to determine optimal concentration in your cell culture system.

Store -10 to -40 Degrees C

References;
1. Band,V. and Sager, R. Proc. Natl. Acad. Sci. USA 86:1249 (1989)
2. Clark, R. Stampler, M.R., Milley, R., O’Rourke, E., Walen, K.H., Kriegler, M., Koplin, J., and McCormick, F. Ca. Res. 48:4689(1988)
3. Hammond, S.L., Ham, R.G., and Stampler, M.R. Proc. Nat. Acad. Sci. USA 81:5435 (1984)

 


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